Another consideration is the choice of promoter. The SV40 promoter was conventionally used until research showed that vectors driven by the Rous Sarcoma Virus (RSV) promoter had much higher expression rates. More recently, expression rates have been further increased by the use of the cytomegalovirus (CMV) immediate early promoter. Inclusion of the Mason-Pfizer monkey virus (MPV)-CTE with/without rev increased envelope expression. Furthermore the CTE+rev construct was significantly more immunogenic than CTE-alone vector.  Additional modifications to improve expression rates have included the insertion of enhancer sequences, synthetic introns, adenovirus tripartite leader (TPL) sequences and modifications to the polyadenylation and transcriptional termination sequences. An example of DNA vaccine plasmid is pVAC, it uses SV40 promoter.
SV40 was the 40th virus found in rhesus monkey kidney cells when these cells were used to make the polio vaccine. This virus contaminated both the Inactivated Polio Vaccine (IPV) created by Dr. Jonas Salk and the Oral or "Live" Polio Vaccine (OPV) created by Dr. Albert Sabin.
Children being fed sugar cubes with the oral polio vaccine. Circa 1961.
In 1961, SV40 was discovered by Dr. Bernice Eddy of the National Institute of Health, Division of Biologics when she took the material used to grow polio vaccines and injected it into hamsters. Tumors grew in the hamsters. Her discovery was subsequently validated by Drs. Maurice Hilliman and Benjamin Sweet of Merck.
Upon the discovery that SV40 was an animal carcinogen that had found its way into the polio vaccines, a new federal law was passed in 1961 that required that no vaccines contain this virus. However, this law did not require that SV40 contaminated vaccines be thrown away or that the contaminated seed material (used to make all polio vaccines for the next four decades) be discarded. As a result, known SV40 contaminated vaccines were injected into children up until 1963. In addition, it has been alleged that there have been SV40-contaminated batches of oral polio vaccine administered to some children until the end of the 1990's.
So what else in these DNA (GMO) vaccines?
After looking at what RSV does to DNA, to say these DNA vaccines affect DNA seems a sizable understatement. RSV gets into a person's DNA where it then gets the person's cell nuclei to reproduce RSV (a sarcoma - a cancer) and rapidly.In 1911, Peyton Rous discovered that cancer could be induced in healthy chickens by injecting them with a cell-free extract of the tumor of a sick chicken.
He ground up samples of the tumor and passed the material through a filter with pores so fine that not even bacteria could get through. However, the tumor filtrate was able to induce cancer when injected into chickens.
This was the first demonstration of an oncogenic virus — that is, a virus capable of causing cancer. The tumor was a sarcoma, a tumor of connective tissue. The virus was named the Rous sarcoma virus (RSV).The Rous sarcoma virus is a retrovirus (as is HIV, the virus that causes AIDS).
As soon as RSV infects a cell, its reverse transcriptase synthesizes DNA copies of its genome. These enter the nucleus of the cell and insert themselves randomly throughout the DNA of the host's chromosomes.
Normal gene transcription within the nucleus now produces an RSV messenger RNA (mRNA) that reenters the cytoplasm. Some copies of this mRNA are then translated by the normal machinery (e.g., ribosomes) of the host cell into protein products. Other copies of the RNA become incorporated into new virus particles.
.... each end of the RNA molecule has a set of repeated sequences of nucleotides ("R" and "P") that perform at least two important functions:
- they enable the DNA copies of RSV to insert into the host's DNA and
- they act as enhancers, causing the host nucleus to transcribe the RSV genes at a rapid rate.
What else is in these DNA (GMO) vaccines - vaccines which have never been approved for human use in the US?
The DNA vaccines also use cytomegalovirus as a promoter. And cytomegalovirus is associated with small heads (and many other severe problems) in babies (see below)r.
The CDC refers to "congenital" cytomegalovirus as CMV that is "present at birth." But DNA vaccines include CMV and logically they might pass this virus to pregnant women and then fetuses could become infected with it. So for a baby to be born with a CMV infection doesn't mean the CMV is "congenital." It could be vaccine-induced. But it's handy to refer to CMV as "congenital" CMV as that immediately distracts from the DNA vaccines (that are not approved for human use) containing it. It also slips past the fact that these vaccines (and they would be DNA vaccines) are being incessantly pushed on pregnant women. So, when a baby is born with a CMV infection, rather than asking what caused the CMV infection and perhaps see this DNA vaccine connection, a baby with the infection is simply considered to have a very unfortunate congenital condition.
From the CDC: http://www.cdc.gov/cmv/congenital-infection.html
Most (90 of every 100) infants who are infected with cytomegalovirus (CMV) at birth (congenital CMV infection) appear healthy at birth. Health problems or disabilities due to congenital CMV infection may appear 2 or more years after birth, or they may never appear—80 of every 100 infants with congenital CMV infection never develop symptoms or disabilities.
Signs and Symptoms
Signs of CMV infection that may be present at birth
- Premature birth
- Liver problems
- Lung problems
- Spleen problems
- Small size at birth
- Small head size
Permanent health problems or disabilities due to congenital CMV infection
- Hearing loss
- Vision loss
- Mental disability
- Small head size
- Lack of coordination
- Death (in rare cases)
Children with congenital CMV infection are more likely to have permanent disabilities if they had symptoms of CMV infection at birth. However, some children with congenital CMV infection who appear healthy at birth can develop hearing or vision loss over time due to congenital CMV infection. For this reason, if you know your baby was born with CMV infection, it is important to have his or her hearing and vision tested regularly.
The mechanism of action of high dose vitamin C is known and understood. In normal healthy tissues it acts as an antioxidant. In other tissues, it generates hydrogen peroxide, the chemical that platinum blondes use to bleach their hair. This happens in sick and inflamed tissues, for example in a malignant tumour. The process is typically a form of Fenton reaction, generating free radicals. The oxidation and free radicals arising from the hydrogen peroxide kill bacteria and inactivate viruses. In other words, vitamin C acts as a targeted bleach and antiseptic. http://orthomolecular.org/resources/omns/v10n13.shtml
Given supplemental ascorbate (vitamin C), not merely from birth but also all throughout gestation, Klenner’s uniformly healthy, trouble-free infants were known by the staff as the “Vitamin C Babies.” (12)
In a 1978 letter to Klenner, Irwin Stone writes that he thinks that “giving levels of ascorbate for long periods of time at the daily levels you recommend. . . is equivalent to creating a new human subspecies, ‘Homo sapiens ascorbicus’ . . . with unusual resistance to disease and stress and with a prolonged life span.”